Bone rnaseq
WebSingle-Cell RNA-Seq Identifies Multiple Cell Populations in the Bone Marrow. The 10× Genomics Chromium platform was employed to construct single-cell RNA-seq libraries … WebApr 1, 2024 · Single-cell RNA sequencing analysis of human bone-marrow-derived mesenchymal stem cells and functional subpopulation identification Zhongyu Xie, Wenhui Yu, Guiwen Ye, Jinteng Li, Guan Zheng,...
Bone rnaseq
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National Center for Biotechnology Information WebApr 27, 2024 · To better understand the changes occurring in the tumor microenvironment during MM progression, we performed single-cell RNA sequencing (RNA-seq; 10x Genomics) on BM aspirates from patients at ...
WebApr 13, 2024 · Consistent with the results we observed in bone aspirates, there were lower MKs in bone tissues of MM patients with platelets less than 120 × 10 9 /L (Fig. 1f), while the number of MKs and ... WebAug 30, 2024 · To perform AEI, we used transcriptome-wide sequencing (RNA-seq) data after QC from articular cartilage (n = 65) and subchondral bone (n = 24) from OA patients. While the cartilage samples were not fully paired between preserved and lesioned (56 preserved and nine lesioned cartilage), all subchondral bone samples were paired (24 …
WebMay 15, 2024 · Applications of RNA-seq on Skeletal Tissues. As an unbiased molecular profiling method, RNA-seq has been applied to a very diverse set of problems in the field … WebObjectives: To characterize MKs and HSCs from human bone marrow using single-cell RNA sequencing, to investigate MK lineage commitment, maturation steps, and thrombopoiesis. Results: We show that MKs at different levels of polyploidization exhibit distinct transcriptional states. Although high levels of platelet-specific gene expression occur ...
WebApr 12, 2024 · Abstract. WHIM Syndrome is a rare immunodeficiency caused by gain-of-function CXCR4 mutations. Here we report a decrease in bone mineral density in 25% of WHIM patients and bone defects leading to ...
WebNov 8, 2024 · In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. plb16f0000WebDec 6, 2024 · We therefore performed a comprehensive assessment of human bone marrow cells using both single-cell RNA sequencing and multiparameter flow cytometry from 20 healthy adult human donors across a broad age range. These data characterize variation between healthy donors as well as age-associated changes in cell population frequencies. prince edward county tax departmentWebMay 17, 2024 · Single-cell RNA-seq in neutrophils is technically feasible. We sorted Ly6G-positive CD11b-positive neutrophils from bone marrow, peripheral blood, and spleen of healthy 6–8-week-old male B6 mice ... prince edward county snow stormWebMesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. prince edward county skating clubWebAccess to space, equipment, and expertise for performing bulk RNA extractions from bone and cartilage tissue, or recovering single cells from these tissues for single cell RNA … prince edward county stormWebApr 14, 2024 · Osteoporosis is a disease that is characterised by reduced bone mineral density (BMD) and can be exacerbated by the excessive bone resorption of osteoclasts … prince edward county taxi serviceWebNov 13, 2024 · Here we have applied single-cell RNA-sequencing (scRNA-seq)to fresh human bone marrow CD34 + cells: we profiled 391 single hematopoietic stem/progenitor … plb2s-c0